recombinant rat pdgfbb  (PeproTech)

Journal: Biology of reproduction

Article Title: Cell-type localization of platelet-derived growth factors and receptors in the postnatal rat ovary and follicle.

doi: 10.1095/biolreprod.105.046854

Figure Lengend Snippet: FIG. 6. Effect of PDGF on in vitro growth of preantral follicles. Preantral follicles of sizes 120–200 lm in diameter were cultured for 5 days in serum-free medium in the presence of various treatments. Cross-sectional diameters of follicles were measured daily and the average percentage increase in follicle diameters of each group at each day compared to Day 0 was determined. Sample sizes for the 5-day culture period were: control n ¼ 7, PDGFAA n ¼ 9, PDGFAB n ¼ 9, PDGFBB n ¼ 6, AG1296 n ¼ 7, and FSH n ¼ 14. *P , 0.05, **P , 0.005.

Article Snippet: The final concentration of treatments were: 100 ng/ml recombinant rat PDGFAA (R&D Systems, Inc., Minneapolis, MN), 100 ng/ml recombinant rat PDGFAB (R&D Systems, Inc., Minneapolis, MN), 100 ng/ml recombinant rat PDGFBB (R&D Systems, Inc., Minneapolis, MN), 15 lM AG1296 (Calbiochem, La Jolla, CA) or 1 I.U./ml recombinant human FSH (National Hormone and Peptide Program, Torrance, CA).

Techniques: In Vitro, Cell Culture, Control